Cellular Delivery |
| Polymer Micro- and Nanoparticles |
Scanning Electron Microscope Image of 2 µm Polystyrene Particles |
Our group is investigating polymeric nano and microparticle based on polystyrene functionalised with an amino group. This opens up a plethora of easy and simple modifications of the particles and therefore we have been demonstrated a variety of different applications.
Polymeric particles from our group are in particular fascinating not only due to their chemical nature of stability without any surfactants but also due to the ability of entering cells without causing harm.
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Donut Particles
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We have synthesised Particles with a broad variety of applications in particular for cells. For example, fluorescent particles for easy cellular analysis (125) and modified particles to measure intracellular pH (163) as well as calcium concentration (142). We also demonstrated that the particles are an easy tool to use for cellular delivery of a wide variety of biological active molecules such as proteins (191) and nucleic acids (187). |
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We have prepared a novel, cell-friendly heterogeneous palladium catalyst that is able to carry out Suzuki-Miyaura cross-coupling reactions under physiological conditions (in vitro) (See picture). Palladium nanoparticles encapsulated on polystyrene microspheres act as a non-toxic catalyst and we are developing exciting new applications ranging from labeling to in situ synthesis of novel drugs. (208) |
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For Publications about Polymeric Nano and Microparticle see below or under Publications |
| Peptoids |
A variety of peptide sequences have been identified to easily cross the cell membrane and deliver attached cargo into cells. However, peptides are not very stable and have a low bioavailibility. To overcome this our group synthesised more stable peptoids (mimicking peptides) which are able to enter cells as well as cellular compartments such as the nucleus. (178)
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| Cationic Lipids |
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Another way to deliver compounds into cells is by employing cationic lipids which can form micelles around the cargo hence trafficking it into cells. These lipids are mainly used for the delivery of nucleic acids such as DNA for gene transfection. We are developing novel cationic lipids and formulations for better gene transfection results. (203) |
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| Recent Cellular Delivery Publications |
(209) Safe and efficient in vitro and in vivo gene delivery: tripodal cationic lipids with programmed biodegradability
A. Unciti-Broceta, L. Moggio, K. Dhaliwal, L. Pidgeon, K. Finlayson, C. Haslett and M. Bradley, Journal of Material Chemistry, 2011, 21, 2154-2158. |
(208) Palladium-mediated Intracellular Chemistry
R. M. Yusop, A. Unciti-Broceta, E. M. V. Johansson, R. M. Sanchez-Martin and M. Bradley, Nature Chemistry, 2011, 3, 241-245. |
(207) Multi-modal Molecular Imaging Approaches to detect primary cells in preclinical models
K. Dhaliwal, L. Alexander, G. Escher, A. Unciti-Broceta, M. Jansen, N. Mcdonald, J. M. Cardenas-Maestre, R. Sanchez-Martin, J. Simpson, C. Haslett and M. Bradley, Faraday Discussions, 2011, 149, 107-114. |
(206) Zeta Potential Mediated Reaction Monitoring on Nano and Microparticles
F.Thielbeer, K. Donaldson and M. Bradley, Bioconjugate Chemistry, 2011, 22, 144–150. |
(203) Strategies for the Preparation of Synthetic Transfection Vectors
A. Unciti-Broceta, M. N. Bacon and M. Bradley, Topics in Current Chemistry, 2010, 296, 15-49. |
(201) Investigation of microsphere-mediated cellular delivery by chemical, microscopic and gene expression analysis
L. M. Alexander, S. Pernagallo, A. Livigni, R. M. Sanchez-Martin, J. M. Brickman and M. Bradley, Molecular BioSystems, 2010, 6, 399-409. |
(191) Microsphere-Mediated Protein Delivery into Cells
Rosario M. Sanchez-Martin, Lois Alexander, Mathilde Muzerelle, Juan M.
Cardenas-Maestre, Anestis Tsakiridis, Joshua M. Brickman and Mark
Bradley, ChemBioChem, 2009, 10, 1453-1456. |
(187) Knocking (Anti)-Sense into Cells: The Microsphere Approach to Gene Silencin
L. M. Alexander, R.M. Sanchez-Martin, M. Bradley, Bioconjugate Chemistry 2009, 20, 422-426. |
(181) Thiol-containing microspheres as polymeric ligands for the immobilization of quantum dots.
J. M. Behrendt, M. Afzaal, L. M. Alexander, M. Bradley, A.V. Hine, D. Nagel, P. O'Brien, K. Presland, A. J. Sutherland, Journal of Materials Chemistry 2009, 19, 215-221. |
(178) Synthesis, Penetrability and Intracellular Targeting of Fluorescein-Tagged Peptoids and Peptide-Peptoid Hybrids
A. Unciti-Broceta, F. Diezmann, C. Y. Ou-Yang, M. A. Fara, M. Bradley, Bioorganic & Medicinal Chemistry, 2009, 17, 959-966. |
(177) Very Long-Chain Fatty Tails for Enhanced Transfection
A. Liberska, A. Unciti-Broceta, M. Bradley, Organic Biomolecular Chemistry, 2009, 7, 61-68. |
(173) Solid-phase synthesis of a lysine-capped bis-dendron with remarkable DNA delivery abilities
How, SE; Unciti-Broceta, A; Sanchez-Martin, RM, and Bradley M., Organic and Biomolecular Chemistry, 2008, 6, 2266-2269 |
(171). Dunking
Doughnuts into Cells? Selective Cellular Translocation and
In Vivo Analysis of Polymeric Micro-Doughnuts
Alexander L, Dhaliwal K, Simpson J, Bradley M, Chemical
Communications 2008, 30, 3507-3509. |
(169) Cellular uptake of fluorescent labelled biotin-streptavidin
microspheres
M. Bradley, L. Alexander and R. M. Sánchez-Martín, Journal of Fluorescence, 2008, 18, 733-739. |
(166) Combining Nebulization-Mediated
Transfection and Polymer Microarrays for the Rapid Determination
of Optimal Transfection Substrates
Unciti-Broceta, Asier; Diaz-Mochon, Juan; Mizomoto,
Hitoshi; Bradley, Mark, Journal of Combinatorial
Chemistry, (2008), 10, 179-184. |
(163) pH sensing in living
cells using fluorescent microspheres
M. Bradley, L. Alexander, K. Duncan, M. Chennaoui,
A. C. Jones, and R.M. Sanchez-Martin, Bioorg. Med. Chem.
Lett. (2008), 18, 313-317. |
(162) Peptoid dendrimers - Microwave-assisted
solid-phase synthesis and transfection agent evaluation
Juan J. Diaz-Mochon, Mario A. Fara, Rosario M. Sanchez-Martin,
Mark Bradley,,
Tet. Lett. (2008), 49, 923-925. |
(151)
Biological cell detection using ferromagnetic microbeads
Mitrelias, T.; Palfreyman, J.; Jiang, Z.; Llandro, J.; Bland, J. A. C.; Sanchez-Martin, R. M.; Bradley, M, Journal of Magnetism and Magnetic Materials (2007), 310, 2862-2864. |
(142) Microsphere-Based Real-Time Calcium Sensing
R. M. Sanchez-Martin, M. Cuttle, S. Mittoo, M. Bradley, Angew. Chem. Int. Ed. (2006), 45, 5472-5474. |
(129) Synthesis and cellular uptake of cell delivering PNA-peptide conjugates
J.J. Diaz-Mochon, L. Bialy, J. Watson, R. M. Sanchez-Martin, M. Bradley, Chem. Comm. (2005), 26, 3316-3318. |
(125) Bead-based cellular analysis, sorting and multiplexing
R.M. Sanchez-Martin, M. Muzerelle, N. Chitkul, S. E. How, S. Mittoo, M. Bradley, ChemBioChem (2005), 6, 1341-1345. |
(120) Functionalized Cross-Linked Microspheres as an Efficient Cellular Delivery
R.M. Sanchez-Martin, M. Muzerelle, N. Chitkul, S. Mittoo, M. Bradley, ChemBioChem (2005), 6(8), 1341-1345. |
(217) Polymerizable Fluorescein Derivatives: Synthesis of Fluorescent Particles and Their Cellular Uptake
F. Thielbeer, S. V. Chankeshwara, M. Bradley, Biomacromolecules (2011) |
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